megatomi.com for Dummies
megatomi.com for Dummies
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Our new Distinct+ tissue clearing approach is the only technique that delipidates samples without any adjust in morphology and with small influence on structural integrity.
Megatome can be a vibrating microtome built to portion a broad range of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With higher blade vibrating frequency and minimized blade deflection, Megatome allows substantial-throughput tissue sectioning with uniform floor profile, together with negligible tissue destruction and information loss.
Antibodies could get weeks to diffuse via only a few millimeters of tissue, having a steep labeling gradient from surface area to core.
Megatome is made for precision: the blade vibrates at the next frequency and bigger amplitude array than other microtomes, and contains a exceptional deflection Regulate system.
SE makes use of a rotational electric field to disperse highly electromobile molecules (which include antibodies or surfactant micelles) all over a porous sample devoid of detrimental electrically charged structures within the tissue. This allows two-four day clearing of intact organs,
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Megatome can be a novel microtome which allows for high-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with small tissue problems and data decline.
eFLASH is usually a quick tissue labeling method which allows for uniform entire-organ staining in twenty rounds of labeling.
Entirely delipidate whole mouse brains or comparably sized samples in only one day with SmartBatch+, or in one 7 days with our passive clearing kit.
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Protect avoids the variability of hydrogel embedding and the information reduction from PFA preservation, shielding specimens for multiple rounds of processing.
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Our novel Defend tissue preservation strategy varieties intramolecular bonds applying polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.